Current research
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Function of neuromedin U in the metastatic process of colon cancer.
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Role of microvesicles released by human colorectal cancer cells HT29 overexpressing transcriptional factor Snail in preparing metastatic niche.
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Formation of cell migration structures.
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Role of the cyclic nucleotide balance regulating Rho- and ROCK- dependent signaling in cell motility.
Routinely used technics:
- Cell culture – primary and continuous cell lines.
- Analysis of gene expression by real time -PCR.
- Analysis of protein expression – SDS/PAGE, immunobloting.
- Flow cytometry.
- ELISA.
- Confocal microscopy - sample preparation and image analysis.
- Cloning and expression of recombinant proteins.
- Stable or transient cell transfection– gene overexpression or silencing.
- Cell proliferation assays and cell function tests (adhesion, migration, invasion, capillary tube formation).
- Analysis of metalloproteinases expression and activity (fluorescent gelatinase zymography assay, quench fluorescence method).
- Isolation of cell microvesicles by differential centrifugation steps.
